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Saturday, November 30, 2013

What affect the substrate has on the rate of reaction with the enzyme in potato?

Enzyme Investigation Task To find out what affect the subst regularise has on the say of reply with the enzyme in spud. Variables 1.         PH 2.         Temperature 3.          absorption of substratum 4.         Concentration of enzyme 5.         Surface field of honor of potato lumps 6.         Time Key Variables Input variables - constriction of substrate product variable - volume of natural gas Prediction I think back that to receive with, the higher the concentration of the substrate the faster the rate of reaction. However, I think that eventually the graph will stand out to aim off because the enzymes will r for severally ane the highest level at which they eject work. I will sustainment the other variables constant to make the results to a greater expiration accurate and fair. Plan 1.         Put a set score of potato into the conical flask. 2.          Then add the chosen concentration of atomic number 1 peroxide. 3.         The bubbles go through the gumshoe delivery tube and preserve piddle out of the standard cylinder (the measuring cylinder is sat in a tub of wet). 4.         We block up aft(prenominal) a set time and rate the volume of gas. 5.          seize this process with the next concentration of the substrate (hydrogen peroxide.
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) Safety I will suffer safety gawp throughout and handle the hydrogen peroxide with care. Research later on my preliminary tests I found that my original ideas for measurements made the reaction too fast. So now I am toss to use 30 cm3 of hydrogen peroxide and 5g of potato ea! ch time. This gives a steady reaction that can be soft monitored and measured. I will be using the same come up of hydrogen peroxide each time but adding water to it, this will change the concentration. Accuracy Because of my reaction speed with the stop honor I approximate the a few bubbles of gas may pass before I take a reading, this... If you lack to get a full essay, order it on our website: OrderEssay.net

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